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MOLECULAR GENETICS
 
INTERNET EXPLORER USSERS - PL. UNABLE THE JAVA SCRIPT TO VISUALIZE THIS PAGE PROPARLY....
"In biology, any study begins with the choice of a 'system.' On this choice depends the experimenter's freedom to maneuver, the nature of the questions he is free to ask, and even, often the type of answer he can obtain."
CELL

Transient expression of hSSB1 protein in Hella cells. Human SSB was fused to two tags Flag and GFP. The expression is driven by CMV constitutive promoter. The images are taken under Delta vision microscope. At list SSB1:GFP appears to be expressed predominantly in the nucleus (see the green channel only). Pre-extraction of the cells doesn’t show SSB to be strongly bound to DNA. No surprise here since SSB is a Single Strand Binding protein. So, what are the functions of hSSB1 and hSSB2 proteins? It is long been accepted that RPA protein is the protein which is the one involved in DNA replication in eukaryotes and plays vital role in DNA repair. In prokaryotes this role is taken by a small protein called SSB. Interestingly SSB is very conservative between species including humans. Recently hSSB1, hSSB2 and hSSB3 ware identified. Reed more....

In the 1980s, Kary Mullis at Cetus Corporation conceived of a way to start and stop a polymerase's action at specific points along a single strand of DNA.  Mullis realized that by harnessing this component of molecular reproduction technology, the target DNA could be exponentially amplified.  For this his discovery Kary B. Mullis, was awarded the 1993 Nobel Prize for chemistry.  So simple is the concept of PCR that many scientists to date think that this is not a real discovery but putting things together. In retrospective PCR is the most important techniques in molecular biology. See PCR applications Site Directed Mutagenesis Page

"Genius is born--not paid. "
Oscar Wilde
 
"The important thing is not to stop questioning. Curiosity has its own reason for existing. One cannot help but be in awe when he contemplates the mysteries of eternity, of life, of the marvelous structure of reality. It is enough if one tries merely to comprehend a little of this mystery every day. Never lose a holy curiosity."
Albert Einstain